منابع مشابه
Initial bridges between two ribosomal subunits are formed within 9.4 milliseconds, as studied by time-resolved cryo-EM.
Association of the two ribosomal subunits during the process of translation initiation is a crucial step of protein synthesis. The two subunits (30S and 50S) of the bacterial 70S ribosome are held together by 12 dynamic bridges involving RNA-RNA, RNA-protein, and protein-protein interactions. The process of bridge formation, such as whether all these bridges are formed simultaneously or in a se...
متن کاملLikelihood-based classification of cryo-EM images using FREALIGN.
We describe an implementation of maximum likelihood classification for single particle electron cryo-microscopy that is based on the FREALIGN software. Particle alignment parameters are determined by maximizing a joint likelihood that can include hierarchical priors, while classification is performed by expectation maximization of a marginal likelihood. We test the FREALIGN implementation using...
متن کاملDescribing geometry and symmetry of cryo-EM datasets using algebra
Cryo-electron microscopy (cryo-EM) is a microscopy technique used to discover the 3D structure of molecules from very noisy 2D images. This thesis presents two projects that use algebra to describe cryo-EM datasets. In the first part, we obtain an algebraic description of common lines datasets. Common lines are lines of intersection between cryo-EM images in 3D. They are an important ingredient...
متن کاملSubunit conformational variation within individual GroEL oligomers resolved by Cryo-EM.
Single-particle electron cryo-microscopy (cryo-EM) is an emerging tool for resolving structures of conformationally heterogeneous particles; however, each structure is derived from an average of many particles with presumed identical conformations. We used a 3.5-Å cryo-EM reconstruction with imposed D7 symmetry to further analyze structural heterogeneity among chemically identical subunits in e...
متن کاملOrientation Determination from Cryo-EM images Using Least Unsquared Deviation
A major challenge in single particle reconstruction from cryo-electron microscopy is to establish a reliable ab-initio three-dimensional model using two-dimensional projection images with unknown orientations. Common-lines based methods estimate the orientations without additional geometric information. However, such methods fail when the detection rate of common-lines is too low due to the hig...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2020
ISSN: 1548-7091,1548-7105
DOI: 10.1038/s41592-020-0925-6